Animal crossings are designed to mitigate barrier effects of transportation infrastructure on wildlife movement. However, many crossings are not placed in optimal locations and may not take into account the animals’ natural movement patterns. Our study, published in PLOS One, calculates the additional distance that Tibetan antelope must travel in order to move across an ecosystem that has been bisected by a railway. Our study suggests that the crossing location can prolong the migration distance of these antelope by >80 km. To better conserve long-distance migration, we advocate for the need for long-term studies to assess wildlife movement prior to the establishment of these anthropogenic barriers.